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White cell cast and WBCs (500). The images below display the microscopic findings. ARTIFACTS AND CONTAMINANTS A variety of foreign objects can find their way into the urine specimen during collection, transportation, while being tested, or while on the microscope slide. These methods include dye-binding, immunochemistry, modified biuret methods, and turbidimetric methods. The tissue's colloidal osmotic pressure (interstitial fluid pressure), along with the capillary's hydrostatic pressure (filtration pressure), regulates the outward flow of fluid from the capillary. Graff's textbook of urinalysis and body fluids 3rd edition pdf document. More than 500 photos and illustrations — 50 new to this edition –clarify concepts and familiarize readers with the normal and abnormal structures found in body fluids. 30 CoA cannot enter the Krebs cycle; therefore, it is diverted to the formation of ketone bodies.
The nonspecific ferric chloride screening test can detect the presence of many amino acids and may still be used for urine testing despite its limitations. Blood flow by dilating the afferent arteriole and constricting the efferent arteriole, by stimulating sodium reabsorption in the proximal convoluted tubule, and by triggering the release of the hormone aldosterone from the adrenal gland and antidiuretic hormone (ADH, and also known as vasopressin) from the pituitary gland. It appeared milky and shimmery. Malignant cells seen in CSF: a. can be of CNS origin b. can be leukemia cells c. Graff's textbook of urinalysis and body fluids 3rd edition pdf pdf. metastasize from other sites d. may be all of these 17. Water enters the system through consumption of either water or food and also through cellular metabolic processes. The percent of viable sperm is reported. Tetrahydroquinoline 23. Appear as pink or red and the source of the blood, whether fetal or maternal, can be distinguished by the KleihauerBetke test for fetal hemoglobin. The urates in this field are clumped close together.
3, 6 Lamellar body counts provide a reliable estimate of fetal lung maturity. 2 mL of fresh semen. Common causes of Fanconi syndrome in children are genetic inborn errors of metabolism. Graff's Textbook of Urinalysis and Body Fluids 3rd –. Overlay the acid with centrifuged urine by allowing. Fat and chyle give urine a milky appearance. They may be convoluted, straight, or curved, and they may vary in length. Lipids may be released from bone marrow after injury to the bone. The most commonly used sample is the 24-hour specimen. Therefore, pericardial effusions are usually always exudates.
Reagent strip method on a urine sample and is confirmed by refrac-. IRIS DIAGNOSTICS DIVISION The Iris iQ® Body Fluid Module adapts the iQ®200 for identification and enumeration of cells in most body fluids including cerebrospinal, pleural, peritoneal, peritoneal lavage, peritoneal dialysate, pericardial, and general serous fluids. Dissolve the copper sulfate in approximately 100 mL of hot water and pour into the citrate–carbonate solution with stirring. Specific gravity is measured by assessing refractive index of LED-emitted light as it passes through the specimen. TUBULOINTERSTITIAL DISEASE. Glucose oxidase methods are more sensitive to solutions of aqueous glucose than to glucose in urine; therefore, they are more sensitive to dilute urine than concentrated urine. Clow CL, Reade TH, Scriver CR.. Graff's textbook of urinalysis and body fluids 3rd edition pdf trader. 5 Historically, the osmolality required more time, expense, and equipment than specific gravity, which is why it has not been included in the routine urinalysis procedure. An advantage of the guaiac tests over the other fecal. 3 Therefore, it is important to interpret the protein result by correlating it with the specific gravity. Serous fluid is an ultrafiltrate of plasma and is maintained by the pressure forces (tissue colloidal osmotic pressure, capillary hydrostatic pressure, capillary colloidal osmotic pressure, and tissue hydrostatic pressure) and by the absorption of fluid into the lymphatic system. In most cases, little more information is added by reporting urine color or appearance in addition to all of the other routine procedures; therefore, some laboratories no longer include these in the regular urinalysis report. The motility is abnormally low which may indicate infertility.
Nitrofurantion contributes a color to urine that may cause misinterpretation of this test. However, in this case the WBC count is low. Chemical tests for blood combined with microscopic examination can usually reveal the cause of red urine. Ampulla of ductus deferens. It will also lower the pH, bringing it closer to the isoelectric point of proteins; therefore, the cloudiness may increase after addition of the acid due to increased precipitation of proteins. In addition, the fluid may cling to the side of the test tube rather than running down to the bottom. X (syphilitic, leptospiral, and unusual organisms, e. g., Listeria). 36 Procedure for Acetest: 1. Figure 11-13 shows a Reiter cell (neutrophil-laded macrophage). ■ High-Complexity Testing (HCT)—These tests require a high degree of interpretative knowledge and skill and must be performed by more experienced personnel and/or more complex instrumentation.
False-Positive Results Most dipsticks will give false-positive results in the presence of certain oxidizing contaminants such as hypochlorites which may be used to clean urine-collection containers. Phase microscopy artificially retards diffracted light by one fourth of a wavelength, and this produces a halo where the surfaces of slightly differing refractile indices meet one another. It is important that the technologist be able to recognize these objects as being extraneous structures. In adults, the kidneys normally excrete levels up to 150 mg/dL (0. The two-drop method will allow for quantitation up to 5% but the "pass-through" phenomenon may still occur when very large concentrations of sugar are present. The process that occurs is a combination of active secretion and plasma ultrafiltration. Talcum powder particles and a few squamous epithelial cells (160). 000 plus the scale reading. An appropriate gauge needle is attached to a syringe and the entry site is cleansed.
↑Urine calcium, kidney stones, ↑blood potassium. Aspiration of joint fluid is indicated for any patient with a joint effusion or inflamed joints. Gastrointestinal Tract Physiology and Fecal Formation 278 Disorders of the Gastrointestinal Tract 278 Fecal Specimen Collection 278 Fecal Analysis Methods 279. Multistix2 (5 RBCs; 0. Protein Electrophoresis Protein electrophoresis is performed on concentrated CSF to identify the type and relative amounts of proteins that may be present in the CSF. Assa S. Evaluation of urinalysis methods used in 35 Israeli laboratories. Hair and a coarsely granular cast. 2 Neutrophils may be vacuolated or contain bacteria or crystals.
National Institute of Diabetes and Digestive and Kidney Disorders. 11/17/09 7:57 PM Page 46 Aptara Inc. 46. and its oxidized variant, urobilin (a brown pigment), are lost in the feces.