TruStrip™ Rapid Tests and Sample Transfer Strips. 2020) "Chronic Presence of Oligomeric Aβ Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression. " Several ELISAs targeting Aβ have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. 800-678-5599. headquarters: USA. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples. ELISA Kits and reagents for Therapeutic mAbs, biosimilars, and proteins. Target: Amyloid beta 42.
Mouse Aβ42(Amyloid Beta 42) ELISA Kit. After appropriate washing steps, an anti-APBB3 detection antibody conjugated to biotin binds to the captured analyte. Inter-Assay: CV<10%. Use only as directed. Sample Type: Cerebrospinal Fluid, 10 µL Supernatant, 10 µL Tissue Homogenate, 10 µL.
TMB substrate is used for color development at 450nm. Por favor conéctese. Waltham, MA USA 02451. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 and is highly specific just to amyloid beta peptide.
Price (USD): Size: Species Reactivity: Mouse, Rat. Combes M, Poindron P, Callizot N. (2015) "Glutamate protects neuromuscular junctions from deleterious effects of β-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture. " Epub ahead of print] Application: Transgenic mouse brain homogenates. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Acta Neuropathol Commun. Ab193692 has been referenced in 1 publication. We will contact you within 24 hours.
While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. DescriptionWe are producing ELISA Kit for research use only. 2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells. " All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. 05% Tween 20 in PBS. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. Detection MethodColorimetric. The Biosensis o-Aβ Elisa detects Aβ oligomers as validated and described by Youmans KL et al (2012) in mouse and by Combes M et al (2015), in rat and human samples. Components 1 x 96 tests 1100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Human Amyloid beta detection antibody 2 vials Human Amyloid beta Standards (lyophilized) 2 vials Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml. Whether you have a product question or require order assistance we are ready to help. Custom peptides & Antibodies. This immunoassay kit allows for the specific measurement of this index in serum, plasma, tissue... Reactivity: - Mouse. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. CategoriesElisa Kit. This is a sandwich ELISA in strip well format with reagents for up to 96 tests. Product DescriptionThe oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer's Disease.
Áreas de investigación. Assay durationMultiple steps standard assay. Aβ40 ELISA Kit allows for the in vitro quantitative determination of Aβ40 concentrations in serum, plasma, tissue homogenates and other biological fluids. Sample typeCell culture supernatant, Serum, Plasma. Expiration Date12 months from purchase. Product Name: Description: Beta amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. IHC/ICC Slides and Lysates for Westerns. Sample Requirements.