When utilized in ELISAs, the oligomeric form of Aβ peptide (o-Aβ) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. TMB substrate is used for color development at 450nm. 1 x 96 Well Plate(s). After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. Assay durationMultiple steps standard assay. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis. " ELISA, Western, LFA, IHC/ICC, and IP reagents. Product name: Amyloid beta 42 Mouse ELISA Kit.
Store the complete kit at 2–8°C. This Mouse APBB3 activity assay is for the quantitative determination of Amyloid Beta Precursor Protein Binding Protein B3 in samples including tissue homogenates and other biological fluids. Other brands: NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience. Chem Soc Rev 49:5446-5472 (2020). Alpha Diagnostic International, All rights reserved. In-vitro screening of drug candidates. Sample Type: Cerebrospinal Fluid, 10 µL Supernatant, 10 µL Tissue Homogenate, 10 µL. Tai, LM (2014) "Amyloid-_ Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. " M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models. " Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary. Alternative NamesBeta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta. Mouse Amyloid Beta Precursor Protein Binding Protein B3 ELISA KitIMSAPBB3KT. Apr 24;12:16 Application: Mouse lysate.
Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols. Cerebrospinal fluid, Tissue extract, Cell culture supernatant. This immunoassay kit allows for the specific measurement of this index in serum, plasma, tissue... 2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells. " For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. Sample Requirements. Cantidad: Referencia EM0864. This is a sandwich ELISA in strip well format with reagents for up to 96 tests. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Your browser version seems rather old, and we don't support it. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology. " Several ELISAs targeting Aβ have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide.
Storage InstructionsStore at 2-8°C. The Biosensis oligomeric Aβ ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric Aβ peptides. This material is not suitable for human or animal use. Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal Aβ aggregation and limits secretion of Aβ via exosomes. " Batch NumberPlease see item label. Please upgrade your browser for the complete experience of our website. Sample typeCell culture supernatant, Serum, Plasma. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. Abcam's Amyloid beta 1-40 Human ELISA Kit (ab193692) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amyloid beta 1-40 in serum, plasma, and cell culture supernatants. Application DetailsELISA. Application: Synthetic constructs. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Ab193692 has been referenced in 1 publication. Storage Temperature2-8°C.
Chemical sensing platforms for detecting trace-level Alzheimer's core biomarkers. Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit are selective to Aβ 42 with insignificant cross-reactivity to Aβ 40.
Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Detection MethodColorimetric. Loading... company name: Invitrogen. Isotype and Serum Controls.
Use only as directed. Price (USD): Size: Species Reactivity: Mouse, Rat. The Biosensis o-Aβ Elisa detects Aβ oligomers as validated and described by Youmans KL et al (2012) in mouse and by Combes M et al (2015), in rat and human samples. Product NameOligomeric Amyloid-beta, Human, Rat, ELISA assay. Availability: In stock. Acta Neuropathol Commun. 2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents. " TargetOligomeric Amyloid-beta. Peptides, Proteins, Enzymes, cDNAs, Aptamers, Adjuvants and ODNs.
Species reactivityReacts with: Human. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm. Kasus-Jacobi A et al. Warning: Last items in stock! Whether you have a product question or require order assistance we are ready to help. 2020) "Chronic Presence of Oligomeric Aβ Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression. " The antibodies have been generated to the N-terminus of Aβ 42.
The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody were generated to ABeta 1-42. Key features and details. 123 nanograms per millilter. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease. " Toll Free: (800)-786-5777 | 210-561-9515 | Fax: 210-569-6105 | Web: Copyright ©1996 - 2023. Colorimetric detection at 450 nm. Target Host SpeciesHuman. Epub ahead of print]. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice. " Application: Tris-extracts of mouse brain homogenates.
Add color reagents, 30 min. Thermo Fisher Scientific81 Wyman Street. Detection antibody: 1 hr. Predicted to work with: Mouse. Availability date: For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number.
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