This assay employs an antibody specific for Human Amyloid beta 1-40 coated on a 96-well plate. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease. Mouse Amyloid Beta Precursor Protein Binding Protein B3 ELISA Kit –. " After appropriate washing steps, an anti-APBB3 detection antibody conjugated to biotin binds to the captured analyte. ¿Olvidaste tu contraseña? The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit are selective to Aβ 42 with insignificant cross-reactivity to Aβ 40. Components 1 x 96 tests 1100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Human Amyloid beta detection antibody 2 vials Human Amyloid beta Standards (lyophilized) 2 vials Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml. Product Name: Description: Beta amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase.
No animal systems were used for their manufacture. More info or order: citations: 7. TruStrip™ Rapid Tests and Sample Transfer Strips. 18(5):414-427 Application: Mouse in-vitro assay, cell culture supernatant. Mouse Amyloid Beta Precursor Protein Binding Protein B3 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Amyloid Beta Precursor Protein Binding Protein B3 in biofluid samples, such as tissue homogenates and other biological fluids. Amyloid beta elisa kit mouse review. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. Questions and comments. Aβ40 ELISA Kit allows for the in vitro quantitative determination of Aβ40 concentrations in serum, plasma, tissue homogenates and other biological fluids.
This product can not be ordered from your electronic catalog. Company information. Storage: 4 o C, 1 year.
Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm. It is based upon a Sandwich assay principle and can be used to detect levels of APP / Beta Amyloid Precursor as low as 0. Alpha Diagnostic International, All rights reserved. A quotation will be created for this reference, would you like to continue? Catalog Number: Category: ELISA Kits. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. We actively enforce our Terms & Conditions, including seeking criminal prosecution where possible. Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis. " Sample 1:2 1:4 1:8 serum(n=5) 87-103% 85-99% 85-101% EDTA plasma(n=5) 82-89% 86-98% 83-101% heparin plasma(n=5) 82-93% 82-96% 83-96%. FroggaBio - Homepage. Combes M, Poindron P, Callizot N. (2015) "Glutamate protects neuromuscular junctions from deleterious effects of β-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture. "
Flow cytometry antibodies. Epub ahead of print]. Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Amyloid beta elisa kit mouse pad. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice. " This is a sandwich ELISA in strip well format with reagents for up to 96 tests. ELISA, Western, LFA, IHC/ICC, and IP reagents. Detection MethodColorimetric. Shipping: Overnight delivery in styrofoam box with ice packs.
Apr 24;12:16 Application: Mouse lysate. Molecular Neurodegeneration 2016 11:35. Price (USD): Size: Species Reactivity: Mouse, Rat. This immunoassay kit allows for the specific measurement of this index in serum, plasma, tissue... In-vitro screening of drug candidates. Privacy Statement |. Mouse endotoxin elisa kit. In this case, make sure that you have filled in the desired quantity. Purchase with confidence on our PCI compliant website.
39 68% - 136% Cell culture media 104. The kit detects amyloid-beta 1-42 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract. Colorimetric detection at 450 nm. Detection antibody: 1 hr.
For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. 3 77% - 125% Plasma 97.
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